牡蛎粗多糖对免疫应激仔猪炎性细胞因子和PPARγ mRNA转录水平的影响

doi:10.11843/j.issn.0366-6964.2014.03.020

Abstract

Abstract:

The present study evaluated whether Oyster Polysaccharides could attenuate immune challenge induced by LPS.A total of thirty Du grown and crossbred castrated piglets of 28±1 d were randomly allocated into five groups (namely Blank control，Immunological stress control and Oyster polysaccharide High，Medium and Low dose group) with six replicates per group according to the principle of similar weight.Piglets fed basal diet supplemented with 0 (Control) or 0.5%，0.8%,1.2% OPS (OPS High，Medium and Low dose group) for 30 days.The piglets were injected intraperitoneally with a dose of Escherichia coli LPS (100 μg·kg^－1 BW) except the Blank control which were injected with the same dose of normal saline.Plasma TNF-α，IL-1β and IL-6 concentrations were measured by ELISA.Piglets were killed for collect liver，spleen，kidney，lymph nodes and thymus，the PPARγ mRNA transcription of them were measured.The results showed that plasma IL-1β，IL-6 and TNF-α level in stress control was significant increased than the other groups （P＜0.05）.Plasma IL-6 and TNF-α level among Low-dose group，Medial-dose group and Blank control were not significantly different（P>0.05），but those were significant decreased compare with High-dose group（P＜0.05）.The OPS-supplemented piglets had highly significant decreased PPARγ mRNA transcription level in Liver，spleen，kidney，and thymus compared with piglets from Stress control（P<0.01），but the OPS-supplemented piglets had greater PPARγ mRNA transcription level in lymph nodes compared with piglets from Stress control.The Low-dose group，and Medial-dose group PPARγ mRNA expression level was closer blank control compare with High-dose group.These results indicate that dietary supplementation of OPS was able to alleviate the immunological stress caused by LPS，and the effect of remission is better between 0.5% to 0.8%.

CLC Number:

S852.4

LUO Gang，HUANG Zhi-jian，CHEN Teng-teng，JIANG He-ji. Effects of Oyster Crude Polysaccharides on Inflammatory Cytokine and PPARγ mRNA Transcription of Weanling Piglets after Immunological Stress[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 483-488.

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Effects of Oyster Crude Polysaccharides on Inflammatory Cytokine and PPARγ mRNA Transcription of Weanling Piglets after Immunological Stress

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